| 1. | After transformation of bl21 with pet - np , an expressed fusion protein with molecular weight of 60ku was identified by sds
经sds page 、 westeth七分析证实表达的重组蛋白具有良好的抗原反应活性。 |
| 2. | So through gene engineering technology , we clone v - src gene into pgex - kt , expressed in e . coli tgi and dh5 a after transformation and iptg induction
因此，我们设想在大肠杆菌中表达v - src蛋白，填补目前研究的一些缺陷。 |
| 3. | Further , it puts forward the technology transformation schema and the transformation schema of the corresponding system , analyses the economic after transformation
并提出了进行全面技术改造方案，相应系统的改造方案。对改造后的经济性进行了分析。 |
| 4. | After transformation of the data model and processing of the thematic mapping elements , the paper constructs a mapping system of the seabed sediment thematic digital map
经过对数据模型的转换以及专题制图要素的处理，建立了海洋底质专题数字图成图系统。 |
| 5. | Research also indicate that explant influence the frequency of callus induction , especially influence embryogenic calli formation . 5 . the proper concentration of ppt is 3 . 0mg / l in screening resistant callus after transformation
在转化后抗性愈伤组织筛选中，适应的ppt筛选浓度为3刀mg几， cef有效抑菌浓度为200pg ml ，乙酚丁香酮有效诱导浓度为100pmol几， 0 |
| 6. | Reconstruction of antisense pcdnas - doc - 1r plasmid after transformation the pmeiss - fl3 - doc - 1r plasmid into e . coli cells , we have extracted the plasmid and cut doc - 1r fragment using xho i to get a 975 bp doc - 1r cdna fragment
Pcdna3 - doc - 1r反义重组质粒的构建将pme18s - fl3 - doc - 1r质粒转化后进行质粒提取。用xhoi限制性内切酶从克隆载体上切取975bp的doc - 1rcdna片段。 |
| 7. | After transformation , we have chosen the positive bacterium clones by bacterium colon pcr . with dna sequencing , we have made sure direction of dna sequence which inserted into the plasmid and have named sense and antisense reconstruction plas - mids respectively
采用sanger双脱氧末端终止法再进一步进行dna序列测定分析，检测doc一1rcdna序列插人表达载体的方向，确定正、反义重组体。 |
| 8. | Though we know the forms of partition function before and after transformation , we will not study directly the property of partition function itself but the transformation which makes it unchanged with the idea of renormalization group
我们虽然利用p - v路方法写出了重整化变换前后配分函数的形式，但并没有直接研究配分函数本身的性质，而采用了重整化群的思想，研究使配分函数保持不变的变换。 |
| 9. | The implementation uses two - dimension images as input . the arrangement of light spots on the lunar rover and the imaging geometry of the camera are used to compute the three - dimension coordinates of the light spots in camera coordinate system , and after transformation , the world coordinate of the light points . we can then easily get the coordinate value of center of mass of the rover after averaging the world coordinate value of light points
本文构造了一种光点配置单目ccd算法,它以计算机视觉系统采集的二维图像信息作为输入,直接利用光点的几何配置条件和摄像机成像几何关系,求解出光点在ccd摄像机坐标系下的三维坐标,并经过坐标变换,转化为在世界坐标系下的坐标,然后取其平均值,从而得出月球车的中心位置。 |
| 10. | The results show that , after transformation the size , shape and distribution of primary silicon in hypereutectic al - si alloy have a serious effect on the tool life and the machined surface quality of the alloy , the alloy whose primary silicon is fine , spread and dull angular has a better machinability
试验结果表明：过共晶铝硅合金经变质处理后组织中初晶硅的大小、形状和分布状况对切削刀具寿命和合金的切削加工表面质量影响极大，具有细小、分散而形状圆钝化初晶硅的合金有比较好的切削性能。 |
